Charles Frankenstein created Eva in the tower room laboratory of his castle out of parts cultivated from the bodies of the dead. The genesis for her creation was that she was to be the mate for Frankenstein's first successful trial in reanimation, the Frankenstein Monster, Viktor. When The Bride was created, the original monster began pawing at his "bride", but Charles, having a change of heart, admonished the creature and drove him away.

After calming the frightened woman down, Frankenstein put her to bed and began crafting a false origin for her. He named her Eva and claimed that she was an amnesiac from a nearby town whom Frankenstein had taken in as his personal ward. Frankenstein viewed Eva as a new breed of woman – one who was every bit the equal of any man.